EMAN2.1 - A New Generation of Software for Validated Single Particle Analysis and Single Particle Tomography
نویسندگان
چکیده
منابع مشابه
Automated Cryo-tomography and Single Particle Analysis with a New Type of Phase Plate
Recent years have shown an increased interest in the development and use of phase plates in cryo-EM. The oldest and the most productive type of phase plate is the carbon film Zernike phase plate [1]. It has been successfully used in cryo-tomography [2] and single particle analysis applications [3]. Despite its good performance the Zernike phase plate has a few pitfalls. One major practical hind...
متن کاملPhase Contrast Cryo-Electron Tomography and Single Particle Analysis with a New Phase Plate
Biological samples are very sensitive to beam irradiation because of induced changes to their structure by the incident electron. Due to the limited allowed dose, cryo-images of frozen-hydrated biological specimens are typically noisy and low in contrast. One way to improve the imaging of such samples is by using a phase plate to increase contrast and signal-to-noise ratio. The improved contras...
متن کاملAutomatic Particle Picking Algorithms for High Resolution Single Particle Analysis
As new genome sequencing initiatives are completed, one of the next great challenges of cell biology is the atomic resolution structure determination of the enormous number of proteins they encode. Single particle analysis is a technique which produces 3D structures by computationally aligning high resolution electron microscope images of individual, randomly oriented molecules. One of the limi...
متن کاملa new algorithm and software of individual life insurance and annuity computation
چکیده ندارد.
15 صفحه اولAlignment error envelopes for single particle analysis.
To determine the structure of a biological particle to high resolution by electron microscopy, image averaging is required to combine information from different views and to increase the signal-to-noise ratio. Starting from the number of noiseless views necessary to resolve features of a given size, four general factors are considered that increase the number of images actually needed: (1) the ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Microscopy and Microanalysis
سال: 2014
ISSN: 1431-9276,1435-8115
DOI: 10.1017/s1431927614005881